CHARDAKOV METHOD PDF

2) In “Chardakov method for water potential measurement” experiment, you find that M solution has not been changed by water loss or absorption by the. Chardakov Technique. Plant Physiology. UNI. Incubating tissue in solution. Pre- incubation. Incubation. Post-incubation. Save for later measurement. Did solution change concentration? Solutes stay the same If water left or entered tissue –Water also entered or left solution –Solution becomes more dilute or.

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Be sure not to include any fragments of the skin. At this point, the water potential of the solution equals the water potential of the potato cores. No change in density, concentration No net water movement Tissue and solution were at the same WP If we can find chardakpv solution concentration —We can find the tissue WP.

Measuring Water Potential by the Gravimetric Technique.

Then remove the tissues, gently blot on paper towels and reweigh. Which method do you think will be more accurate? Work quickly to minimize evaporation and keep the tissue wrapped in a moist towel. Chardakov Method for Determining Water Potential. The answer will metthod in Jm -3 which are metyod to pressure Pa.

Alternately, for a more accurate measurement of changes in the solution density, a refractometer can be used. Auth with social network: Cytology Osmosis and Water relations. Weigh the cores to the nearest 0.

Chardakov method

Pour off the solutions into a set of empty, correspondingly labeled tubes. Water potential values determined methof this method may be slightly more negative than those obtained by the Chardokov method.

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Incubate the cores for at least 1. Change in Weight final – initial g. Cut them to the same length ca. Two ways to move water Bulk flow Osmosis Both move water from high energy to low Differences —Source of energy difference.

Investigating osmosis: measuring the water potential of a potato cell

Incubate the cores for 1. Record your data in Metyod 2. Cell Transport The movement of molecules can be either passive no energy or active needs energy depending cchardakov the membrane structure and concentration. Use the following equation to calculate the percent change in weight for each tissue by the following equation: Be sure to use a different pipet for each dye stock.

What would happen to The size of the tissue? What is the water potential of potato tissue?

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In contrast to the Chardakov method which analyzes changes in solution density after incubation, this technique monitors tissue weight changes. Determine the approximate sucrose concentration for which there is no net change in density after tissue incubation i. Registration Forgot your password? Boy, Oh Buoyancy Does it Float? Draw the chardajov fit line for your data.

This occurs when the apoplast becomes infiltrated with water and solutes. Repeat for all solutions. Published by Sabrina Grant Modified over 2 years ago. My presentations Profile Feedback Log out. Response of drops float, sink, hover when placed in solutions in which potato cores have been incubated.

If the density of a solution does not change no net movement of water then this solution has the same water potential as the tissues that were incubated in it. Dispense 10 mL of water or a sucrose solution 0. Osmosis diffusion of water through a chadrakov permeable membrane Membrane X is permeable to water but not to protein Which side has the highest concentration.

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Feedback Privacy Policy Feedback. Immerse the pipette in the water that previously had tissue sections in it until the tip is approximately at the center of the tube. From the metod, determine the concentration of the sucrose solution in which there was no net weight gain i.

Plant Physiology

Then, the percent change in weight of the tissue is plotted versus solution concentration or osmotic mehod. About project SlidePlayer Terms of Service. An alternate method to determine his point requires performing a regression analysis of the best fit line of your data.

Do the cores show various degrees of turgor? If you wish cjardakov download it, please recommend it to your friends in any social system. Cut them to the same length with a razor blade ca. Incubating tissue in solution Pre-incubationIncubation Post-incubation Save for later measurement. Hypertonic Concentration with higher solute concentration and less water concentration Hypotonic lower solute concentration and more water fhardakov.

One distinct advantage of this technique is that it provides a more accurate estimate of water potential. Record the temperature of the solutions Table 1 Using a Pasteur pipet, remove a small amount of water dyed with methylene blue to dye the sucrose solution, dip a dry probe into methylene blue powder and then mix.